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KMID : 1094020080250050355
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Journal of Veterinary Clinics
2008 Volume.25 No. 5 p.355 ~ p.358
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Protective Effect of Trophic Factor Supplementation on Cold Ischemia/Rewarming Injury to Kidney Cells
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Kwon Young-Sam
Jang Kwang-Ho
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Abstract
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The aim of this study was to investigate whether trophic factor supplementation (TFS) enhance the survival of kidney cell during cold ischemic storage and rewarming. The effect of TFS on the phosphorylation of p44/42 and p38 mitogen activated protein kinases (MAPK) signaling pathway was determined by Western blot. Apoptotic changes after cold ischemic storage and rewarming was determined by 4’,6’-diamino-2-phenylindole (DAPI) staining. The cell viability was evaluated by live assay. TFS significantly decreased p44/42 and p38 MAPK activity induced by cold ischemic injury and rewarming (p < 0.05). The number of apoptotic cells was decreased after 5 minute rewarming in the presence of TFS. TFS significantly increased the cell viability after 5 minute rewarming (p < 0.05). Therefore, it was concluded that trophic factor supplementation protects kidney tubule cells from cold ischemic and rewarming injury via the inhibition of p44/42 and p38 MAPK activation and reducing apoptotic change.
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KEYWORD
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trophic factors, kidney cells, cold ischemia
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